Supplementary Figures S1-S10 from The Notch Pathway Inhibits TGFβ Signaling in Breast Cancer through HEYL-Mediated Crosstalk

Supplementary Figures S1-S10. Tests for specificity of the anti-HEYL antibody (S1); Notch pathway induces HEYL expression (S2); In vitro analysis of direct interaction of HEYL and Smad3 in Cos 1 cells (S3); Transcriptional control of TGF-ß responsive reporter consisting of tandem synthetic Smad-binding elements by luciferase assays (S4); Hematoxylin and eosin (H&E) staining of mammary glands of wild-type or transgenic HEYL+ virgin mice, 13 weeks of age (S5); Mammary gland H&E staining of wild-type or transgenic mice at day 10 of pregnancy (S6); Mammary gland hyperplasia was commonly observed in HEYL+ transgenic mice, Representative photomicrographs from mouse numbers, 1-4 are shown (S7); Expression of HeyL in mammary tumors from two HEYL+ transgenic mice measured by RT-PCR and IHC staining (S8); Expression of downstream effectors of TGFß, PAI-1 and P15 in HEYL+ transgenic mammary tumors in comparison to normal mammary glands of WT mice (S9); The antagonistic network between Notch and TGF-ß signaling through the interactions between HEY genes and Smads (S10).