Supplementary Figure 4 from Endoneurial Macrophages Induce Perineural Invasion of Pancreatic Cancer Cells by Secretion of GDNF and Activation of RET Tyrosine Kinase Receptor

PDF file - 174K, Expression of the GDNF receptors GFRa1 and RET in specimens excised from patients with pancreatic ductal adenocarcinoma.(a) Low magnification (X20) immunofluorescence staining with anti-RET ab (green, Alexa 485), (b) anti-GFRa1 ab (red, Alexa 565) , and (c) overlay image with DAPI staining (blue). (d) Immunohistochemical staining of the same specimen with anti-CK19 ab (brown staining). (e) High magnification (X40) fluorescent microscope imaging performed with anti-RET (green, Alexa 485), (f) anti-GFRa1 antibodies (red, Alexa 565) and (g) DAPI (blue staining). The overlay image in (h) shows co-expression of the receptors by PDA cells.