Proteins in the insulin-secreting cell line MIN6 bind the imidazoline compound BL11282.

The imidazoline BL11282 stimulates insulin release and alters islet proteomes. Subcellular fractions of MIN6 cells showed that the membrane fraction exhibited binding to BL11282 on a Biacore chip and to BL11282-labelled magnetic beads. Bound material extracted from the beads showed a ∼50kDa differential band upon SDS–PAGE and a weaker 100kDa band. The former was sensitive to competitive removal by preincubation of the fraction with BL11282, then highlighting the ∼100kDa band. Masspectrometric analysis revealed the ∼50kDa band to be EF1A and the ∼100kDa band to be glucose regulated P94, both of interest in insulin synthesis and secretion.